Coding

Part:BBa_K4759041:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


hemA-RBS2-hemL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1264
    Illegal EcoRI site found at 2321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1264
    Illegal EcoRI site found at 2321
    Illegal NheI site found at 2031
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1264
    Illegal EcoRI site found at 2321
    Illegal BglII site found at 246
    Illegal BglII site found at 524
    Illegal BamHI site found at 1258
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1264
    Illegal EcoRI site found at 2321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1264
    Illegal EcoRI site found at 2321
    Illegal AgeI site found at 1681
    Illegal AgeI site found at 2014
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


Design Notes

Connect the hemA and hemB via RBS2 for transcription under the control of the same promoter


Source

E.coli genome

References

[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.